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Discovery of the Largest Spider Web in Sulfur Cave

11/20/2025, 4:40:43 PM

Unprecedented Spider Colony in Sulfur Cave

Researchers have uncovered what is believed to be the largest spider web on record, measuring approximately 1,140 square feet (106 square meters) in Sulfur Cave, located on the border between Albania and Greece. This remarkable finding, reported in the journal *Subterranean Biology*, marks the first documented instance of cave spiders constructing webs collectively. The web was initially discovered in 2022 by cavers from the Czech Speleological Society, situated in a permanently dark area of the cave, about 164 feet (50 meters) from the entrance.

The colony consists of over 100,000 spiders, predominantly made up of 69,000 *Tegenaria domestica*, commonly known as the barn funnel weaver or domestic house spider, and more than 42,000 *Prinerigone vagans*, a type of sheet weaver. Notably, both species are not typically known for communal living, with *T. domestica* usually found in human habitats and *P. vagans* inhabiting wet environments like meadows and marshes.

Unique Living Conditions

The extreme conditions of Sulfur Cave, characterized by high sulfur gas concentrations due to a sulfidic stream, create a harsh environment that surprisingly supports a diverse ecosystem, including worms, beetles, scorpions, and fish. The researchers hypothesize that the lack of light within the cave allows *T. domestica*, which is generally more aggressive, to coexist with *P. vagans*. The spiders may not perceive each other as threats, leading to a unique symbiotic relationship where they share the web instead of preying on one another.

Dietary Adaptations and Microbiome Changes

The spiders' diet primarily consists of non-biting midges that thrive on microbial biofilms produced by sulfur-oxidizing bacteria. This diet has been shown to affect the spiders' microbiomes, resulting in decreased gut bacterial diversity compared to their counterparts living outside the cave.

Methodology and Density Estimates

To estimate the spider population density, researchers employed a systematic approach by counting funnel-shaped webs in designated quadrants. They acknowledged that their methodology might lead to overestimations due to the presence of abandoned webs, which can be challenging to differentiate from active ones. Despite this potential overcount, the findings suggest a significant density of spiders within the cave.

Implications of the Discovery

The discovery of this massive spider web not only challenges existing notions about spider behavior and communal living but also highlights the adaptability of species in extreme environments. The Sulfur Cave serves as a unique ecological niche, raising questions about the interactions between species and their adaptations to harsh conditions.

Verbatim Quotes

  • “The methodology used to estimate spider density may lead to an overestimation due to the presence of abandoned funnel webs that are difficult to distinguish from those in use.” — Researchers from the study.