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High-speed footage reveals how honey-bee stingers keep pumping venom after the bee dies

By Drooid · · How we work

New footage uncovers the double-piston mechanism

Researchers led by postdoctoral fellow Fiorella Ramirez Esquivel at Macquarie University filmed a western honey-bee (Apis mellifera) stinger penetrating an artificial skin substitute at hundreds of frames per second. The videos show two valves moving oppositely, a “double piston” that drives the barbed stinger deeper while continuously pumping venom from the sac into the wound. The motion was reproduced with 3D-printed models.

Evolutionary context and colony defense

Western honey-bee workers are sterile; their sole evolutionary interest is protecting the colony, a “superorganism.” The barbed stinger’s ability to stay embedded and keep delivering venom after the bee’s death is an example of autotomy—leaving part of the body behind as a defensive weapon. Professor Juliana Rangel (Texas A&M) describes the stinger as an “insurance policy” for the colony.

Key researchers

  • Fiorella Ramirez Esquivel – lead author, Macquarie University
  • Juliana Rangel – professor of apiculture, Texas A&M University
  • Lauren Ponisio – associate professor, University of Oregon

Mechanics and timing

  • The stinger remains active for roughly two minutes after detachment.
  • Valve twitches can continue for up to 15 minutes, delivering venom long after the initial sting.
  • Imaging at 100 fps captured alternating inflation and collapse of the pistons, a motion likened to a “reciprocating saw” that both advances the stinger and pumps fluid.

Potential applications

The dual-piston design is unlike any commonly observed natural or engineered pump. Researchers suggest the principle could inspire micro-pumps for medical devices that require a continuous, controlled flow of liquid, as well as other compact, self-sustaining pumping mechanisms.

Official statements & responses

Ramirez Esquivel emphasized that the stinger’s action differs from a conventional syringe, which delivers a single, fixed volume. The two-plunger system enables a continuous flow of venom, a feature potentially valuable for novel fluid-delivery technologies.

Rangel noted that the barbs and sustained venom delivery increase the likelihood of deterring larger predators, reinforcing the colony’s defensive strategy. She added that most other stinging insects lack barbed stingers because their workers must survive to reproduce, highlighting the unique pressure on sterile honey-bee workers.

Ponisio referenced archaeological evidence of honey harvesting dating back about 9,000 years and expressed curiosity about why other honey-producing bees lack similar stingers.

Verbatim Quotes

  • “Being able to actually film them in action was a brand new thing that is only really possible thanks to modern cameras,” — Fiorella Ramirez Esquivel
  • “Now imagine that you have two of them, and they have to be moving past each other when they go past,” — Ramirez Esquivel
  • “We have known forever [about the stinger and its parts] because we have been the victims of these stinging events for tens of thousands of years," Rangel said.” — Juliana Rangel
  • “This stinging structure and the evolution of the stinging apparatus …” — Juliana Rangel
  • “It's not stopping and starting like a … syringe. The plunger for a syringe, it goes down once and that's it,” — Ramirez Esquivel